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无细胞蛋白合成实验小贴士

时间:2024年06月05日信息来源:本站原创点击:

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参考文献:

1. Liu, WQ., Ji, X., Ba, F. et al. Cell-free biosynthesis and engineering of ribosomally synthesized lanthipeptides. Nat Commun 15, 4336 (2024). https://doi.org/10.1038/s41467-024-48726-y.

2. Gu YQ, Fan F, Liu Y, et al. Cell-free protein synthesis system for bioanalysis: Advances in methods and applications. TrAC-Trend Anal Chem. 2023 161: 117015.

3. Yue K, Chen J, Li Y, Kai L. Advancing synthetic biology through cell-free protein synthesis. Comput Struct Biotechnol J. 2023 4, 2899-2908.

4. Garenne D, Haines MC, Romantseva EF, et al. Cell-free gene expression. Nat Rev Methods Primers 1. 2021 49.

5. Ji XY, Liu WQ, Li J. Recent advances in applying cell-free systems for high-value and complex natural product biosynthesis. Curr Opin Microbiol. 2022 67, 102142.

6. Smolskaya S, Logashina YA, Andreev YA. Escherichia coli extract-based cell-free expression system as an alternative for difficult-to-obtain protein biosynthesis. Int J Mol Sci. 2020 21, 928.

7. Kimura-Soyema T, Shirouzu M, Yokoyama S. Cell-free membrane protein expression. Methods Mol Biol. 2014 1118, 267–73. 

8. 刘晚秋,季向阳,许慧玲,卢屹聪,李健*. 限制性内切酶的无细胞快速制备研究. 合成生物学, 2023, 4, 840–851.

9. Liu WQ, Wu C, Jewett MC, et al. Cell-free protein synthesis enables one-pot cascade biotransformation in an aqueous-organic biphasic system. Biotechnol. Bioeng. 2020 117, 4001-4008.

10. Si Y, Kretsch AM, Daigh LM, et al. Cell-free biosynthesis to evaluate lasso peptide formation and enzyme-substrate tolerance. J Am Chem Soc. 2021 143, 5917-5927.

11. Nuti N, Rottmann P, Stucki A, et al. A multiplexed cell-free assay to screen for antimicrobial peptides in double emulsion droplets. Angew Chem Int Ed Engl. 2022 61, e202114632.

12. Sugiyama S, Yamada K, Denda M, et al. CF-PPiD technology based on cell-free protein array and proximity biotinylation enzyme for in vitro direct interactome analysis. Sci Rep. 2022 12,10592.

13. Hunt AC, Vögeli B, Hassan AO, et al. A rapid cell-free expression and screening platform for antibody discovery. Nat Commun. 202314, 3897.

14. Pardee K, Green AA, Takahashi MK, et al. Rapid, low-cost detection of Zika virus using programmable biomolecular components. Cell. 2016 165, 1255-1266.

15. Voyvodic PL, Pandi A, Koch M, et al. Plug-and-play metabolic transducers expand the chemical detection space of cell-free biosensors. Nat Commun. 2019 10, 1697.

16. Hunt JP, Zhao EL, Free TJ, et al. Towards detection of SARS-CoV-2 RNA in human saliva: A paper-based cell-free toehold switch biosensor with a visual bioluminescent output. N Biotechnol. 2022 66, 53-60.

17. Silverman AD, Karim AS, Jewett MC. Cell-free gene expression: an expanded repertoire of applications. Nat Rev Genet. 2020 21, 151-170. 


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